PyRosetta is available for Mac (64-bit OS X v10.5+) and Linux (64-bit Red Hat and 64-bit Ubuntu) platforms in continuous release versions (usually we have a fresh builds available daily). Running on Windows-10 (thought Unix layer) is are also supported. Additionally, both Python 2.7 and 3.5 is supported.

A Rosetta license is required in order to download and use PyRosetta. Licensing is free for academic and non-profit institutions and is available to commercial users for a fee. Academic and commercial licensing of PyRosetta is handled with the standard Rosetta license through Rosetta Commons. Please click here for more information.

Please use our forums for technical support and assistance or if you have any questions or problems with installing PyRosetta, or consult this download tutorial.

PyRosetta Build Information:

Speed optimized build, use it for production runs on system with no memory constraints.

Build optimized to reduce memory footprint. Use this build with low-memory systems.

Binaries compiled in debug mode with additional asserts enabled and with debug-info compiled-in. Use this to build for debugging.

Python-x.x versions
On CentOS and on Ubuntu Linux we provide binaries for both Python-2.7 and Python-3.5.

Current PyRosetta Versions


Mac OS X 10.7 "Lion/MountainLion" (64-bit) 


Scientific/Red Hat Linux (64-bit)

Ubuntu 16.04 "Xenial" (64-bit)

Windows 10 

PyRosetta-4 Linux build work natively thought Win10 Linux layer. For installation details please see: Instructions for Installing PyRosetta on Windows 10

PyRosetta Binaries in Git repositories use this if you want to frequently update PyRosetta version. (To upgrade simply run 'git pull' on checked out repository. Please note that Git history automatically truncated to store only ~2 last revisions to save disk space.)

To checkout repository with PyRosetta binaries use following command line (replacing 'login' with your user name):

git clone http://login@git-repository-address

So for example Mac command line will be:

% git clone

In order to update these repositories, you must use:

% git fetch && git reset --hard origin/master && git clean -fd

For a full list of available git repositories please see: [PyRosetta-4, Git repositories] (you will need to enter your user name and password to access this page)

Older PyRosetta Versions

Binaries for older PyRosetta releases could be downloaded here: [PyRosetta3 Download]

System Requirements

Build Support Information

PyRosetta is currently only supported on the platforms listed above. Users have sometimes been successful at installing PyRosetta on other platforms; please see the forums for more information, for example, the topic: Building PyRosetta for Linux Mint.


Python 2.7 or 3.5 for 64-bit Linux

Mac OS X

v10.6+ and any Intel-based chipset Mac


Windows-10 or later

Installation Instructions

GNU/Linux and Mac OS X

  1. Obtain a Rosetta license from to receive a username and password.
  2. Download the appropriate version of PyRosetta from the links above.
  3. Unpack the downloaded file to the location of your choice to create the PyRosetta directory.
    (From a terminal/console window, you can unpack the archive using the command: tar -vjxf PyRosetta-<version>.tar.bz2
  4. From within the new PyRosetta directory, type cd setup && sudo python install into the command line to set up the PyRosetta.
  5. Start Python.
  6. In Python, you should be able to import the PyRosetta library with the command import pyrosetta; pyrosetta.init().
    (If this step does not produce a complaint or error, your installation has been successful.)


  1. Obtain a Rosetta license from to receive a username and password.
  2. Follow the [Instructions for Installing PyRosetta on Windows 10]
Subpages (1): PyRosetta3 Download